In vitro evaluation by DAPI staining of fresh, cooled and vitrified donkey embryos
نویسندگان
چکیده
منابع مشابه
Impact of in vitro fertilization by fresh and frozen semen on developmental competence and cryotolerance of buffalo embryos
Production of high quality embryos in vitro needs an efficient in vitro fertilization (IVF). Seminal origin is one of the important factors that affects the success of in vitro embryo production. So our goal was to determine the effect of using fresh and frozen semen in fertilization on developmental competence and cryo-survival of buffalo embryos. Buffalo oocytes wer...
متن کاملEffect of Fresh and Frozen Semen on in vitro Fertilization and Subsequent Development of Goat Embryos
This study was undertaken to compare the efficiency of fresh and frozen-thawed buck semen on in vitro fertilization (IVF) of goat oocytes. Cumulus oocytes complexes (COCs) were collected by aspiration of 2-6 mm diameter follicles, which were obtained from slaughterhouse. Upon grading, only normal quality COCs were maturated in TCM-199 for 48 hours. The percentage of COCs reached to the M-II sta...
متن کاملEffect of vitrification procedure on chromosomal status of embryos achieved from vitrified and fresh oocytes
background: In order to assess the chromosomal status in embryos obtained from vitrified and fresh donated oocytes, preimplantational genetic diagnostic (PGD) was performed after biopsy of one blastomere at day 3. METHODS: A total of 249 oocytes were obtained from 23 oocyte donors, 80 oocytes were used in the vitrified group and 151 oocytes were used in the fresh group. Nine chromosomes (13, 15...
متن کاملexamining the relationship between fear of negative evaluation and communication strategies by iranian efl learners
the purpose of the present study was to investigate the relationship between fear of negative evaluation (fne) and communication strategies (css) among iranian efl learners. it was aimed to examine the differences in the use of communication strategies between speakers with high or low degree of fear of negative evaluation. the current study was a case study consisting of 10 english learners at...
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ژورنال
عنوان ژورنال: Journal of Equine Veterinary Science
سال: 2016
ISSN: 0737-0806
DOI: 10.1016/j.jevs.2016.04.061